An integrated transcriptomic cell atlas of human neural organoids
Cite
He, Z., Dony, L., Fleck, J.S., Szałata, A., Li, K.X., Slišković, I., Lin, H.C., Santel, M., Atamian, A., Quadrato, G. and Sun, J., 2024. An integrated transcriptomic cell atlas of human neural organoids. Nature, 635(8039), pp.690-698. https://doi.org/10.1038/s41586-024-08172-8
Abstract
Human neural organoids, generated from pluripotent stem cells in vitro, are useful tools to study human brain development, evolution and disease. However, it is unclear which parts of the human brain are covered by existing protocols, and it has been difficult to quantitatively assess organoid variation and fidelity. Here we integrate 36 single-cell transcriptomic datasets spanning 26 protocols into one integrated human neural organoid cell atlas totalling more than 1.7 million cells. Mapping to developing human brain references shows primary cell types and states that have been generated in vitro, and estimates transcriptomic similarity between primary and organoid counterparts across protocols. We provide a programmatic interface to browse the atlas and query new datasets, and showcase the power of the atlas to annotate organoid cell types and evaluate new organoid protocols. Finally, we show that the atlas can be used as a diverse control cohort to annotate and compare organoid models of neural disease, identifying genes and pathways that may underlie pathological mechanisms with the neural models. The human neural organoid cell atlas will be useful to assess organoid fidelity, characterize perturbed and diseased states and facilitate protocol development.
Datasets
1. The Human Neural Organoid Atlas
ExploreSource data
Assay type differentiation
Cell line
GM
State exact
Publication
Source data DOI
Cell type level 1
Cell type level 2
Cell type level 3 (rev2)
Cell type
Tissue region (rev2)
Cell type (ntt_rev2)
Tissue type
Subject ID
Assay
Disease
Sex
Tissue
Ethnicity
Age
Velasco, 2019 (doi: 10.1038/s41586-019-1289-x)790295 cells Yoon, 2019 (doi: 10.1038/s41592-018-0255-0)213860 cells Lancaster, 2014 (doi: 10.1038/nprot.2014.158)192720 cells Fiorenzano, 2021 (doi: 10.1038/s41467-021-27464-5); standard78940 cells Quadrato, 2017 (doi: 10.1038/protex.2017.049)65256 cells Jo et al., 2016 (doi: 10.1016/j.stem.2016.07.005)59980 cells Qian et al., 2016 (doi: 10.1016/j.cell.2016.04.032)49275 cells Bhaduri, 2020 (doi: 10.1038/s41586-020-1962-0); most directed45755 cells Bhaduri, 2020 (doi: 10.1038/s41586-020-1962-0); directed43308 cells Watanabe, 2017 (doi: 10.1016/j.celrep.2017.09.047)27874 cells Huang, 2021 (doi: 10.1016/j.stem.2021.04.006)26693 cells Pasca, 2015 (doi: 10.1038/nmeth.3415)20247 cells Miura, 2020 (doi: 10.1038/s41587-020-00763-w)19011 cells Pellegrini, 2020 (doi: 10.1126/science.aaz5626); hChPO15577 cells Trujillo, 2019 (doi: 10.1016/j.stem.2019.08.002)14903 cells Quadrato, 2023 (doi: no_doi)14285 cells Fiorenzano, 2021 (doi:10.1038/s41467-021-27464-5); silk+laminin14130 cells Fiorenzano, 2021 (doi: 10.1038/s41467-021-27464-5); silk14052 cells Andersen, 2020 (doi: 10.1016/j.cell.2020.11.017)13217 cells Pellegrini, 2020 (doi: 10.1126/science.aaz5626); hCO12214 cells Xiang, 2019 (doi: 10.1016/j.stem.2018.12.015)10568 cells Esk, 2020 (doi: 10.1126/science.abb5390)8599 cells Qian, 2020 (doi: 10.1016/j.stem.2020.02.002)6381 cells Birey, 2017 (doi: 10.1038/nature22330)4506 cells Andersen, 2020 (doi: 10.1016/j.cell.2020.11.017); without DAPT3486 cells Sawada, 2020 (doi: 10.1038/s41380-020-0844-z)1441 cells Marton, 2019 (doi: 10.1038/s41593-018-0316-9)28 cells custom_Mito 210229590 cells custom_409b2-iCas982121 cells custom_GIH6-E1160238 cells custom_GIH7-B1257966 cells custom_GIH7-F0247692 cells custom_Mito 29439062 cells HPSI0214i-wibj_225987 cells HES-3 NKX2.1GFP/w10568 cells HPSI0114i-rozh_58599 cells HPSI0314i-hoik_16904 cells HPSI0214i-kucg_24414 cells HPSI0314i-sojd_33929 cells custom_8858-1_8858-3_2242-1_1205-426 cells ARID1B_Mito294_1m23839 cells SUV_Mito210_1m28d23047 cells ARID1B_Mito210_1mr121294 cells SUV_Mito210_3mr119584 cells CHD8_HUES66_6m18336 cells WT MECP2 expression. Fused MGE and cortical organoids.18318 cells CHD8_HUES66_3mr217658 cells ARID1B_Mito210_1mr215445 cells SUV_Mito294_1m15223 cells ARID1B_Mito210_3m14480 cells SUV_Mito210_1m35d12619 cells Non-fused organoids11306 cells Subject age: 27 years. No additional diagnoses. IQ: 1209745 cells WT MECP2 expression. Pooled MGE and cortical organoids prior to fusion.9556 cells SUV_Mito210_3mr27998 cells Sliced organoids6381 cells Mito_wt_harmonized5691 cells Subject age: 27 years. No additional diagnoses. IQ: n/a3676 cells adapted differentiation protocol without DAPT3486 cells Human oligodendrocyte-enriched brain oeganoids28 cells Paulsen, 2022301114 cells Uzquiano, 2022263827 cells Bhaduri, 2020223453 cells Velasco, 2019156220 cells Treutlein, 2023109255 cells Fiorenzano, 2021107122 cells Samarasinghe, 202127874 cells Pellegrini, 202025033 cells Andersen, 202016703 cells Trujillo, 201914903 cells 10.1038/s41586-021-04358-6301114 cells 10.1016/j.cell.2022.09.010263827 cells 10.1038/s41586-020-1962-0223453 cells 10.1016/j.cell.2021.07.003202090 cells 10.1038/s41586-019-1289-x156220 cells no_doi_treutlein109255 cells 10.1038/s41467-021-27464-5107122 cells 10.1038/s41592-021-01344-882121 cells 10.1038/s41586-019-1654-978491 cells 10.1038/s41586-022-05279-832108 cells 10.1038/s41593-021-00906-527874 cells 10.1016/j.stem.2021.04.00626693 cells 10.1126/science.aaz562625033 cells 10.1038/s41587-020-00763-w19011 cells 10.1016/j.cell.2020.11.01716703 cells 10.1016/j.stem.2019.08.00214903 cells 10.1016/j.stem.2023.11.01314285 cells 10.1038/s41591-020-1043-913421 cells 10.1038/s41592-018-0255-011770 cells 10.1038/nature2233011306 cells 10.1016/j.stem.2018.12.01510568 cells 10.15252/embj.20221111188599 cells 10.1016/j.stem.2020.02.0026381 cells 10.1038/s41586-021-04330-42758 cells 10.1038/s41380-020-0844-z1441 cells 10.1038/s41593-018-0316-928 cells 10.1016/j.neuron.2017.07.03526 cells Neural progenitor cells (NPC)528142 cells Neuroepithelium90531 cells Choroid plexus (CP)77315 cells Mesenchymal cell (MC)49267 cells Intermediate progenitor cell (IP)15421 cells Neural crest derivatives9708 cells Oligodendrocyte progenitor cell (OPC)8068 cells Endothelial cell (EC)2451 cells Pluripotent stem cell (PSC)2187 cells Dorsal Telencephalic Neuron517956 cells Non-telencephalic NPC257654 cells Non-telencephalic Neuron225229 cells Dorsal Telencephalic NPC224080 cells Ventral Telencephalic Neuron111988 cells Neuroepithelium90531 cells Choroid plexus (CP)77315 cells Mesenchymal cell (MC)49267 cells Ventral Telencephalic NPC46408 cells Dorsal Telencephalic IP15421 cells Oligodendrocyte progenitor cell (OPC)8068 cells Endothelial cell (EC)2451 cells Pluripotent stem cell (PSC)2187 cells Dorsal Telencephalic Neuron517956 cells Dorsal Telencephalic NPC224080 cells Ventral Telencephalic Neuron111988 cells Neuroepithelium90531 cells Cerebellar NPC58735 cells Medulla Neuron48474 cells Ventral Telencephalic NPC46408 cells Cerebellar Neuron40821 cells Hypothalamic Neuron36346 cells Thalamic Neuron34622 cells Dorsal Midbrain NPC23396 cells Dorsal Midbrain Neuron21096 cells Dorsal Telencephalic IP15421 cells Ventral Midbrain NPC12102 cells Ventral Midbrain Neuron10352 cells Hypothalamic NPC8863 cells Dorsal Telencephalic Neuron NT-VGLUT517956 cells Dorsal Telencephalic neural progenitor cells (NPC)224080 cells Ventral Telencephalic Neuron NT-GABA111988 cells Medulla neural progenitor cells (NPC)95919 cells Neuroepithelium90531 cells Choroid plexus (CP)77315 cells Cerebellar neural progenitor cells (NPC)58735 cells Mesenchymal cell (MC)49267 cells Ventral Telencephalic neural progenitor cells (NPC)46408 cells Thalamic neural progenitor cells (NPC)38746 cells Medulla Neuron NT-VGLUT33798 cells Thalamic Neuron NT-VGLUT27108 cells Cerebellar Neuron NT-GABA26693 cells Dorsal Midbrain neural progenitor cells (NPC)23396 cells Hypothalamic Neuron NT-GABA22643 cells Pons neural progenitor cells (NPC)19893 cells Pons Neuron NT-VGLUT19753 cells Dorsal Midbrain Neuron NT-VGLUT15482 cells Dorsal Telencephalic intermediate progenitor cell (IPC)15421 cells Hypothalamic Neuron NT-VGLUT13703 cells Ventral Midbrain neural progenitor cells (NPC)12102 cells Neural crest derivatives9708 cells Cerebellar Neuron NT-VGLUT9486 cells Hypothalamic neural progenitor cells (NPC)8863 cells Medulla Neuron NT-GLY8719 cells Oligodendrocyte progenitor cell (OPC)8068 cells Ventral Midbrain Neuron NT-VGLUT7628 cells Thalamic Neuron NT-GABA7514 cells Pons Neuron NT-GABA5755 cells Dorsal Midbrain Neuron NT-GABA5614 cells Pons Neuron NT-GLY5341 cells Medulla Neuron NT-GABA5087 cells Cerebellar Neuron NT-GLY4642 cells Ventral Midbrain Neuron NT-GABA2493 cells Endothelial cell (EC)2451 cells Pons Neuron NT-CHOL2317 cells Pluripotent stem cell (PSC)2187 cells Medulla Neuron NT-CHOL588 cells Pons Neuron NT-SER352 cells Medulla Neuron NT-SER282 cells Ventral Midbrain Neuron NT-GLY231 cells Dorsal telencephalon757457 cells Ventral telencephalon158396 cells Dorsal midbrain44492 cells Ventral midbrain22454 cells custom_Mito 210229590 cells Fiorenzano_culture107122 cells custom_409b2-iCas982121 cells custom_GIH6-E1160238 cells custom_GIH7-B1257966 cells custom_GIH7-F0247692 cells custom_Mito 29439062 cells HPSI0214i-wibj_225987 cells HES-3 NKX2.1GFP/w10568 cells HPSI0114i-rozh_58599 cells HPSI0314i-hoik_16904 cells HPSI0214i-kucg_24414 cells HPSI0314i-sojd_33929 cells custom_0410-1-smartseq27 cells custom_8858-1_8858-3_2242-1_1205-4-smartseq26 cells custom_1205-4-smartseq1 cells BD Rhapsody Whole Transcriptome Analysis30511 cells telencephalon802509 cells cerebral cortex353081 cells midbrain tegmentum156397 cells midbrain tectum59980 cells arcuate nucleus of hypothalamus26693 cells ventral part of telencephalon23517 cells choroid plexus15577 cells dorsal plus ventral thalamus10568 cells African American13879 cells blastula stage242590 cells newborn stage (0-28 days)6381 cells 2. HNOCA Extended: The Human Neural Organoid Atlas
ExploreSource data
Assay type differentiation
Cell line
GM
State exact
Treatment
Publication
Source data DOI
Cell type level 1
Cell type level 2
Cell type level 3 (rev2)
Cell type level 4 (rev2)
Tissue region (rev2)
Cell type (ntt_rev2)
HNOCA core
Cell type (extended)
Tissue type
Subject ID
Assay
Disease
Sex
Tissue
Ethnicity
Age
Velasco, 2019 (doi: 10.1038/s41586-019-1289-x)790295 cells Yoon, 2019 (doi: 10.1038/s41592-018-0255-0)213860 cells Lancaster, 2014 (doi: 10.1038/nprot.2014.158)192720 cells Fiorenzano, 2021 (doi: 10.1038/s41467-021-27464-5); standard78940 cells Quadrato, 2017 (doi: 10.1038/protex.2017.049)65256 cells Jo et al., 2016 (doi: 10.1016/j.stem.2016.07.005)59980 cells Qian et al., 2016 (doi: 10.1016/j.cell.2016.04.032)49275 cells Bhaduri, 2020 (doi: 10.1038/s41586-020-1962-0); most directed45755 cells Bhaduri, 2020 (doi: 10.1038/s41586-020-1962-0); directed43308 cells Watanabe, 2017 (doi: 10.1016/j.celrep.2017.09.047)27874 cells Huang, 2021 (doi: 10.1016/j.stem.2021.04.006)26693 cells Pasca, 2015 (doi: 10.1038/nmeth.3415)20247 cells Miura, 2020 (doi: 10.1038/s41587-020-00763-w)19011 cells Pellegrini, 2020 (doi: 10.1126/science.aaz5626); hChPO15577 cells Trujillo, 2019 (doi: 10.1016/j.stem.2019.08.002)14903 cells Quadrato, 2023 (doi: no_doi)14285 cells Fiorenzano, 2021 (doi:10.1038/s41467-021-27464-5); silk+laminin14130 cells Fiorenzano, 2021 (doi: 10.1038/s41467-021-27464-5); silk14052 cells Andersen, 2020 (doi: 10.1016/j.cell.2020.11.017)13217 cells Pellegrini, 2020 (doi: 10.1126/science.aaz5626); hCO12214 cells Xiang, 2019 (doi: 10.1016/j.stem.2018.12.015)10568 cells Esk, 2020 (doi: 10.1126/science.abb5390)8599 cells Qian, 2020 (doi: 10.1016/j.stem.2020.02.002)6381 cells Birey, 2017 (doi: 10.1038/nature22330)4506 cells Andersen, 2020 (doi: 10.1016/j.cell.2020.11.017); without DAPT3486 cells Sawada, 2020 (doi: 10.1038/s41380-020-0844-z)1441 cells Marton, 2019 (doi: 10.1038/s41593-018-0316-9)28 cells custom_Mito 210229590 cells custom_409b2-iCas982121 cells custom_GIH6-E1160238 cells custom_GIH7-B1257966 cells custom_GIH7-F0247692 cells custom_Mito 29439062 cells HPSI0214i-wibj_225987 cells HES-3 NKX2.1GFP/w10568 cells HPSI0114i-rozh_58599 cells HPSI0314i-hoik_16904 cells HPSI0214i-kucg_24414 cells HPSI0314i-sojd_33929 cells custom_8858-1_8858-3_2242-1_1205-426 cells ARID1B_Mito294_1m23839 cells SUV_Mito210_1m28d23047 cells ARID1B_Mito210_1mr121294 cells SUV_Mito210_3mr119584 cells CHD8_HUES66_6m18336 cells WT MECP2 expression. Fused MGE and cortical organoids.18318 cells CHD8_HUES66_3mr217658 cells ARID1B_Mito210_1mr215445 cells SUV_Mito294_1m15223 cells ARID1B_Mito210_3m14480 cells SUV_Mito210_1m35d12619 cells Non-fused organoids11306 cells Subject age: 27 years. No additional diagnoses. IQ: 1209745 cells WT MECP2 expression. Pooled MGE and cortical organoids prior to fusion.9556 cells SUV_Mito210_3mr27998 cells Sliced organoids6381 cells Mito_wt_harmonized5691 cells Subject age: 27 years. No additional diagnoses. IQ: n/a3676 cells adapted differentiation protocol without DAPT3486 cells Human oligodendrocyte-enriched brain oeganoids28 cells Paulsen, 2022301114 cells Uzquiano, 2022263827 cells Bhaduri, 2020223453 cells Velasco, 2019156220 cells Treutlein, 2023109255 cells Fiorenzano, 2021107122 cells Lopez-Tobon, 202329585 cells Samarasinghe, 202127874 cells Pellegrini, 202025033 cells Andersen, 202016703 cells Trujillo, 201914903 cells Giandomenico, 201913280 cells 10.1038/s41586-021-04358-6301114 cells 10.1016/j.cell.2022.09.010263827 cells 10.1038/s41586-020-1962-0223453 cells 10.1016/j.cell.2021.07.003202090 cells 10.1038/s41586-019-1289-x156220 cells no_doi_treutlein109255 cells 10.1038/s41467-021-27464-5107122 cells 10.1038/s41587-024-02157-894129 cells 10.1038/s41592-021-01344-882121 cells 10.1038/s41586-019-1654-978491 cells 10.1038/s41586-022-05279-832108 cells 10.1126/sciadv.adh272629585 cells 10.1038/s41593-021-00906-527874 cells 10.1016/j.stem.2021.04.00626693 cells 10.1126/science.aaz562625033 cells 10.1038/s41587-020-00763-w19011 cells 10.1016/j.cell.2020.11.01716703 cells 10.1101/2024.01.21.57653216114 cells 10.1016/j.stem.2019.08.00214903 cells 10.1016/j.stem.2023.11.01314285 cells 10.1038/s41591-020-1043-913421 cells 10.1038/s41593-019-0350-213280 cells 10.1038/s41592-018-0255-011770 cells 10.1038/nature2233011306 cells 10.1016/j.stem.2018.12.01510568 cells 10.15252/embj.20221111188599 cells 10.1016/j.stem.2020.02.0026381 cells 10.1038/s41586-021-04330-42758 cells 10.1038/s41380-020-0844-z1441 cells 10.1038/s41593-018-0316-928 cells 10.1016/j.neuron.2017.07.03526 cells Neuroepithelium90531 cells Dorsal Telencephalic Neuron517956 cells Non-telencephalic NPC257654 cells Non-telencephalic Neuron225229 cells Dorsal Telencephalic NPC224080 cells Ventral Telencephalic Neuron111988 cells Neuroepithelium90531 cells Ventral Telencephalic NPC46408 cells Dorsal Telencephalic IP15421 cells Dorsal Telencephalic Neuron517956 cells Dorsal Telencephalic NPC224080 cells Ventral Telencephalic Neuron111988 cells Neuroepithelium90531 cells Cerebellar NPC58735 cells Medulla Neuron48474 cells Ventral Telencephalic NPC46408 cells Cerebellar Neuron40821 cells Hypothalamic Neuron36346 cells Thalamic Neuron34622 cells Dorsal Midbrain NPC23396 cells Dorsal Midbrain Neuron21096 cells Dorsal Telencephalic IP15421 cells Ventral Midbrain NPC12102 cells Ventral Midbrain Neuron10352 cells Hypothalamic NPC8863 cells Dorsal Telencephalic Neuron NT-VGLUT517956 cells Dorsal Telencephalic NPC224080 cells Ventral Telencephalic Neuron NT-GABA111988 cells Neuroepithelium90531 cells Cerebellar NPC58735 cells Ventral Telencephalic NPC46408 cells Medulla Neuron NT-VGLUT33798 cells Thalamic Neuron NT-VGLUT27108 cells Cerebellar Neuron NT-GABA26693 cells Dorsal Midbrain NPC23396 cells Hypothalamic Neuron NT-GABA22643 cells Pons Neuron NT-VGLUT19753 cells Dorsal Midbrain Neuron NT-VGLUT15482 cells Dorsal Telencephalic IP15421 cells Hypothalamic Neuron NT-VGLUT13703 cells Ventral Midbrain NPC12102 cells Cerebellar Neuron NT-VGLUT9486 cells Hypothalamic NPC8863 cells Medulla Neuron NT-GLY8719 cells Ventral Midbrain Neuron NT-VGLUT7628 cells Thalamic Neuron NT-GABA7514 cells Pons Neuron NT-GABA5755 cells Dorsal Midbrain Neuron NT-GABA5614 cells Pons Neuron NT-GLY5341 cells Medulla Neuron NT-GABA5087 cells Cerebellar Neuron NT-GLY4642 cells Ventral Midbrain Neuron NT-GABA2493 cells Pons Neuron NT-CHOL2317 cells Medulla Neuron NT-CHOL588 cells Pons Neuron NT-SER352 cells Medulla Neuron NT-SER282 cells Ventral Midbrain Neuron NT-GLY231 cells Dorsal telencephalon757457 cells Ventral telencephalon158396 cells Dorsal midbrain44492 cells Ventral midbrain22454 cells Dorsal Telencephalic Neuron595352 cells Non-telencephalic neural progenitor cells (NPC)282530 cells Non-telencephalic Neuron242235 cells Dorsal Telencephalic neural progenitor cells (NPC)237498 cells Ventral Telencephalic Neuron114070 cells Neuroepithelium90652 cells choroid plexus (CP)80323 cells Mesenchymal cell (MC)49582 cells Ventral Telencephalic neural progenitor cells (NPC)46943 cells Dorsal Telencephalic intermediate progenitor cell (IP)15783 cells Oligodendrocyte progenitor cell (OPC)12231 cells Neural crest derivatives9734 cells Endothelial cell (EC)2478 cells Pluripotent stem cell (PSC)2187 cells custom_Mito 210229590 cells Fiorenzano_culture107122 cells custom_409b2-iCas982121 cells custom_GIH6-E1160238 cells custom_GIH7-B1257966 cells custom_GIH7-F0247692 cells custom_Mito 29439062 cells HPSI0214i-wibj_225987 cells HES-3 NKX2.1GFP/w10568 cells HPSI0114i-rozh_58599 cells HPSI0314i-hoik_16904 cells HPSI0214i-kucg_24414 cells HPSI0314i-sojd_33929 cells custom_8858-1_8858-3_2242-1_1205-426 cells BD Rhapsody Whole Transcriptome Analysis30511 cells telencephalon802509 cells cerebral cortex476795 cells midbrain tegmentum156397 cells midbrain tectum59980 cells arcuate nucleus of hypothalamus26693 cells ventral part of telencephalon23517 cells choroid plexus15577 cells dorsal plus ventral thalamus10568 cells African American13879 cells blastula stage307071 cells newborn stage (0-28 days)6381 cells Source data
Age (days)
Sex
Disease
Subject ID
Publication
Is control
Assay
Batch in data
Cell type level 1
Cell type level 2
Tissue region (rev)
Tissue region (rev2)
Leiden clustering
GSE146878_SC_FXS314766 cells GSE146878_SC_CTRL311241 cells GSM4848890_PTHS-86466 cells hOrg190111_Org25139 cells GSM4848887_PTHS-54764 cells GSM5844877_EML14055 cells GSM4848888_PTHS-63792 cells hOrg180905-Org13773 cells hOrg190111_Org13665 cells GSM4473314_SC_CTRL23588 cells GSM4848885_PTHS-33544 cells GSM4848883_PTHS-13492 cells GSM4848884_PTHS-23368 cells GSM5844876_Control3180 cells GSM4848889_PTHS-73066 cells GSM4848886_PTHS-42944 cells GSM4473313_SC_CTRL12676 cells GSM4473315_SC_FXS12547 cells 206287_rnacount2337 cells 206290_rnacount1948 cells GSM4473316_SC_FXS21739 cells 206282_rnacount1607 cells 206288_rnacount1491 cells 206289_rnacount1391 cells 206286_rnacount1193 cells 206285_rnacount1037 cells myotonic_dystrophy9597 cells neuronal_heterotopia4544 cells pallial_parent10404 cells GABAergic-enriched_patient6858 cells GABAergic-enriched_parent6466 cells treated_pallial_patient4764 cells pallial_patient2944 cells Szebenyi, 2021151064 cells Neuroepithelium7886 cells Dorsal Telencephalic Neuron143733 cells Non-telencephalic neural progenitor cell (NPC)56532 cells Non-telencephalic Neuron29434 cells Mesenchymal cell (MC)16549 cells Dorsal Telencephalic neural progenitor cell (NPC)16062 cells Ventral Telencephalic Neuron16005 cells Ventral Telencephalic neural progenitor cell (NPC)10662 cells Choroid plexus (CP)9935 cells Oligodendrocyte progenitor cell (OPC)8066 cells Neuroepithelium7951 cells Pluripotent stem cell (PSC)2811 cells Neural crest derivatives861 cells Dorsal Telencephalic intermediate progenitor cell (IP)832 cells Endothelial cell (EC)469 cells Dorsal telencephalon161411 cells Ventral telencephalon26842 cells Dorsal midbrain4185 cells Ventral midbrain1524 cells Dorsal telencephalon161243 cells Ventral telencephalon26853 cells Dorsal midbrain3118 cells Ventral midbrain935 cells 
