Transcriptional profile of the rat cardiovascular system at single-cell resolution
Cite
Arduini, A., Fleming, S.J., Xiao, L., Hall, A.W., Akkad, A.D., Chaffin, M.D., Bendinelli, K.J., Tucker, N.R., Papangeli, I., Mantineo, H. and Flores-Bringas, P., 2025. Transcriptional profile of the rat cardiovascular system at single-cell resolution. Cell Reports, 44(1). https://doi.org/10.1016/j.celrep.2024.115091
Abstract
We sought to characterize cellular composition across the cardiovascular system of the healthy Wistar rat, an important model in preclinical cardiovascular research. We performed single-nucleus RNA sequencing (snRNA-seq) in 78 samples in 10 distinct regions, including the four chambers of the heart, ventricular septum, sinoatrial node, atrioventricular node, aorta, pulmonary artery, and pulmonary veins, which produced 505,835 nuclei. We identified 26 distinct cell types and additional subtypes, with different cellular composition across cardiac regions and tissue-specific transcription for each cell type. Several cell subtypes were region specific, including a subtype of vascular smooth muscle cells enriched in the large vasculature. We observed tissue-enriched cellular communication networks, including heightened Nppa-Npr1/2/3 signaling in the sinoatrial node. The existence of tissue-restricted cell types suggests regional regulation of cardiovascular physiology. Our detailed transcriptional characterization of each cell type offers the potential to identify novel therapeutic targets and improve preclinical models of cardiovascular disease.
Datasets
Subject ID
Sex
Disease
Assay
Tissue
Sample ID
LV region
SCVI batch
Clustering (louvain1p2)
Clustering (louvain1p5)
Clustering (leiden1p0)
Clustering (leiden1p2)
Clustering (leiden1p5)
Cell type
Cell type (leiden1p2 - short labels)
Age
Sample preservation method
10x 3' v2, single-nucleus504,278 cells heart left ventricle141,901 cells atrioventricular node58,434 cells heart right ventricle52,379 cells sinoatrial node49,501 cells interventricular septum46,485 cells pulmonary vein46,152 cells left cardiac atrium32,358 cells right cardiac atrium26,812 cells pulmonary artery20,419 cells A_LA3_pipeline_v212,401 cells A_LV13_1B_pipeline_v29,733 cells A_LV11_3B_pipeline_v29,327 cells A_RV1_2G_pipeline_v29,292 cells A_LV11_2A_pipeline_v28,829 cells A_LV7_2B_pipeline_v28,785 cells A_RV2_2G_pipeline_v28,785 cells Sept_NBase_1_pipeline_v28,651 cells A_LV14_1B_pipeline_v28,636 cells A_LV14_2C_pipeline_v28,392 cells A_LV13_3B_pipeline_v28,364 cells Sept_NBase_2_pipeline_v28,234 cells A_LV13_2C_pipeline_v28,140 cells A_RV3_2G_pipeline_v27,971 cells A_RV7_2G_pipeline_v27,908 cells A_LV11_2C_pipeline_v27,704 cells Sept_Apex_2_pipeline_v27,686 cells Sept_NBase_3_pipeline_v27,673 cells A_RA3_pipeline_v27,599 cells A_AV1a_pipeline_v27,589 cells A_LV6_2B_pipeline_v27,569 cells A_LV3_2B_pipeline_v27,453 cells A_AV1d_pipeline_v27,252 cells A_LA6_pipeline_v27,233 cells A_RV5_2G_pipeline_v27,233 cells Sept_Apex_1_pipeline_v27,221 cells A_LV14_2A_pipeline_v27,135 cells A_RA6_12_pipeline_v27,099 cells A_LV4_2B_pipeline_v27,070 cells Sept_Apex_3_pipeline_v27,020 cells A_LA2_pipeline_v26,967 cells A_LV5_2B_pipeline_v26,756 cells A_RV6_2G_pipeline_v26,529 cells Aorta_4_06072019_pipeline_v26,520 cells A_AV2d_pipeline_v26,455 cells A_AV1c_pipeline_v26,438 cells A_RA2_pipeline_v26,415 cells Aorta_2_06072019_pipeline_v26,360 cells A_AV1b_pipeline_v26,307 cells A_SA2c_pipeline_v26,159 cells A_SA2a_pipeline_v26,144 cells A_AV2a_pipeline_v26,131 cells Pulmonary_veins_2a_pipeline_v26,097 cells A_AV2b_pipeline_v26,097 cells A_AV2c_pipeline_v26,058 cells A_LV11_1B_pipeline_v26,033 cells A_LV1_2B_pipeline_v26,028 cells A_LV2_2B_pipeline_v25,923 cells A_SA2d_pipeline_v25,919 cells A_LA8_pipeline_v25,757 cells A_RA8_pipeline_v25,699 cells A_SA1c_pipeline_v25,321 cells A_SA1b_pipeline_v25,141 cells A_LV14_3B_pipeline_v25,022 cells A_LV13_2A_pipeline_v25,002 cells A_SA2b_pipeline_v24,815 cells A_RV4_2G_pipeline_v24,661 cells A_Aorta7_pipeline_v24,501 cells Aorta_3a_06072019_pipeline_v24,453 cells Pulmonary_veins_1a_pipeline_v24,422 cells Pulmonary_veins_1b_pipeline_v24,277 cells Aorta_3b_06072019_pipeline_v24,257 cells Pulmonary_veins_2b_pipeline_v23,831 cells A_Aorta5_pipeline_v23,746 cells A_SA1d_pipeline_v22,770 cells 0: Fibroblasts 184,142 cells 1: Endothelial Cells 176,793 cells 2: Fibroblasts 253,171 cells 3: Ventricular Cardiomyocytes41,978 cells 4: Fibroblasts 333,191 cells 5: Endothelial Cells 232,470 cells 6: Macrophages28,363 cells 9: Atrial Cardiomyocytes20,632 cells 10: Vascular Smooth Muscle Cells 116,646 cells 11: Endothelial Cells 313,383 cells 12: Brown Adipocytes8,840 cells 13: Lymphatic Endothelial Cells7,157 cells 14: Mesothelial Cells6,497 cells 15: White Adipocytes6,496 cells 19: Vascular Smooth Muscle Cells 22,363 cells 21: Top2a+ Endothelial Cells1,533 cells 22: Antigen Presenting Cells1,239 cells 24: Ganglionic plexi neurons891 cells 25: Fmod+ Fibroblasts814 cells 26: Non-myelinating Schwann Cells367 cells 24: G.Plexi Neur891 cells Alias names
SCP2828, GSE280111
